       |
"Many of these studies, however, have used techniques for measuring
platelet aggregation with methodological limitations-either they
depended on platelets already having released their products or having
aggregated, or they involved extensive preparation of plasma
before assessment of aggregability.
Because of these limitations, we have been using ex vivo whole blood
impedance platelet aggregometry to assess the effects of various
conditions on platelet activations. This method makes it possible to
study platelet function in whole blood within a few minutes of
withdrawal of the sample from the patient. It also avoids the complex
processing of blood samples that is required, for example, in
preparation of platelet-rich plasma and thus is more likely to
represent the in vivo status of platelets at the time the blood sample
was obtained."
"The contribution of blood cells to the antiplatelet activity of drugs
shows the importance of using whole blood instead PRP in in
vitro platelet aggregation studies, because this permits a better
extrapolation of the results to in vivo situations."
"...whole blood aggregometry is a simple, fast, and precise method of
determining blood clotting and the effects of drugs in a medium
reflecting almost physiological conditions."
"The whole blood impedance aggregometer is a suitable routine
screening test of platelet dysfunction and inhibition.- Several
drawbacks of the optical system are eliminated. The results can be
obtained more rapidly."
Whole blood aggregation permits "the study of platelet aggregability
soon after blood collection and without any manipulation of the
sample, thus avoiding the loss of red and white blood cells and the
artificial selection of larger platelet populations caused by the
preparation of PRP....the impedance aggregometer has the capability
to detect the early formation of small platelet aggregates...." HERE'S WHAT LAB DIRECTORS SAY ABOUT DIAGNOSTIC USE OF THE WHOLE BLOOD LUMI-AGGREGATION SYSTEM
"...invaluable in working up pediatric patients due to the small
sample volume required ... release by measurement
rather than inference is a tremendous aid to diagnosis."
"...permits rapid diagnosis of platelet release defects and
storage pool disorders within one hour, a vast improvement
in diagnostic accuracy and a 70% reduction in turnaround
time... diagnosis of heparin-dependent antiplatelet antibody
and von Willebrand's disease ...... reliable and accurate
diagnosis and rapid turnaround time, particularly as
limitations in the value of template skin bleeding time and
prevalence of platelet storage pool defects are being
recognized."
"...diagnostic testing for von Willebrands...testing for
hyperaggregability...help confirm a case of Bernard Soulier
Syndrome in an infant, where using an aggregometer with PRP
could not...demonstrate and help explain prolonged bleeding
times in patients on medications other than aspirin or anti-
inflammatories."
"... permits study of platelets in their physiologic milieu of
whole blood, without the centrifugation and specimen
handling required to produce platelet-rich plasma ... a whole
blood lumi-aggregometer which combines the impedance
aggregometry technology with luminescent detection of ATP
secretion is now being employed in clinical laboratories
throughout the world."
The Chrono-log line of aggregometers, reagents and supplies provides a single source for all your platelet aggregation needs. For further information, fill out and transmit our Electronic Reply Card or e-mail your request today. You can also call us at 1-800-CHRONOLOG (1-800-247-6665) or fax us at 610-853- 3972. |
© 1998, 2000 Chrono-log Corporation